C4zip ❲HOT❳

Using a high-organic solvent to release the purified protein directly onto a MALDI plate or into a fresh tube. Real-World Application: Mass Spectrometry

Allows for desalting and concentrating samples in a single, fast step. Standard Workflow for Sample Prep Using a high-organic solvent to release the purified

While C18 is the "gold standard" for peptide mapping, it can bind proteins so tightly that they become nearly impossible to elute. The shorter carbon chain of the C4 resin offers: The shorter carbon chain of the C4 resin

Produced by Millipore , C4 Zip-tips are 10 µL pipette tips containing a specialized C4 (butyl) stationary phase resin. Unlike the more common C18 tips used for peptides, the C4 resin is optimized for larger, more hydrophobic molecules like intact proteins and large polypeptides. Why C4 over C18? Preparing the resin with a wetting solution (e

Preparing the resin with a wetting solution (e.g., 50:50 acetonitrile in water).