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The manual is typically organized into sections covering qualitative and quantitative analyses:
: Includes tables for reagent preparation and normal reference values for various biological fluids.
: Includes protocols for Paper Chromatography , Electrophoresis , and the Glucose Tolerance Test (GTT) .
: Analysis of body fluids such as urine (normal and abnormal constituents), cerebrospinal fluid (CSF), gastric juice, and bile.
: Covers reactions and identification of biological macromolecules including carbohydrates (monosaccharides, disaccharides, polysaccharides), proteins (albumin, gelatin, casein), lipids, and non-protein nitrogenous (NPN) substances.
: Detailed procedures for estimating blood glucose, serum urea, creatinine (including clearance tests), uric acid, total cholesterol, and bilirubin.