This episode marks the official beginning of the in the anime. Key highlights and plot developments include:
: This episode is the first to use the iconic opening "One Day" by The ROOTLESS.
The primary feature of , titled "Ticking Down to the Time of Battle! The Navy's Strongest Lineup in Position!", is the world-shaking revelation that Portgas D. Ace is the biological son of the Pirate King, Gol D. Roger .
: While the execution countdown nears its end, Luffy and the escapees from Impel Down find themselves stuck behind the closed Gates of Justice.